Both location and environment information are the key reference for either buying parties or selling parties in real estate market, however consumers acquired them hardly. It will provide consumers the information, by objectivity and quantity, through photo-realistic cyber city based on Web 3D GIS. Due to large amount of geo-spatial data, narrow network bandwidth, limited computation ability of server, and non-superior performance of client, it is so difficult to perform the photo-realistic cyber city on Web 3D GIS server. Thus, at the beginning, a multi-scale building modeling based on GIS 3D modeling tool is proposed in this study. The degree of scale for a certain building is decided according to its importance and relative location to a major road. The result showed the geo-data volume was decreased largely, and the efficiency during data transfer on the Internet is increased, and the cost in production was also reduced, and therefore its applicability in Web-based 3D GIS is raised. In real estate applications based on the designed Web-based 3D GIS platform, a location and physical environment query system to assist users to choose the optimum house has been thus established. It applied buffer-zone analysis tool on location queries, and other GIS spatial analysis tools, i.e. shadow, view-shed, terrain-profile and sea-level rising analysis, on physical environmental queries. The result showed users can obtain the location and physical environment information of real estate in quantity via this query system.