Forest canopy closure is an important index on ecological study and forest management. Forest canopy closure is closely related to erosion control, water and soil conservation, watershed runoff estimation, which is also used to assess wildlife habitat and forest biomass. In the early years, aerial photography were usually used to interprete forest canopy closure. However after the launch of Landsat-l in 1972, using remote sensing image to estimate forest canopy closure is widely studied. The study area of this research locates at Chi-Tou, Experimental Forest of National Taiwan University. A Landsat Thematic Mapper scene of this area was used in the study. In this study, geometric correction and radiometric correction were done first to improve the quality of the TM imagery. After preprocessing, seventy-two plots with a size of 3*3 pixels were drawn from the TM imagery. Fourteen vegetation indices including TMl、TM2、TM3、TM4、TM5、TM7、GVI、NDVI、R43、R54、R32、VI43、VI45、VI42. Those indices were used as input data for the fuzzy classification approach and the minimum distance pattern classification approach to estimate forest canopy closure, and the results of these two approaches were compared.